Efeitos bioquímicos e histológicos da administração do extrato aquoso de baccharis trimera em ratos submetidos a protocolo de indução dietética de Esteatose Hepática

Abstract

Nonalcoholic fatty liver disease is characterized by a range of disorders that can lead to morphological and functional changes in hepatocytes, ranging from steatosis to steatohepatitis, may progress to cirrhosis and hepatocellular carcinoma. The pathogenesis of the disease involves an imbalance between production and degradation and / or export of triglycerides. Diagnosis is made by imaging methods, biochemical tests and liver biopsy, which is considered the gold standard. Treatment usually involves treatment of comorbidities, through strategies and medications for weight loss, lipid-lowering and insulin sensitizers, and alternative therapies such as herbal medicine, easy access feature and low cost, characterized by the treatment of diseases through use of natural substrates of botanical origin. This dissertation presents two articles: a literature review about the use of herbal medicines in non-alcoholic fatty liver disease and a second article, which aimed to analyze the biochemical and histological effects of administration of the aqueous extract of trimera baccharis (gorse), in rats submitted dietary protocol induction of hepatic steatosis. The latter refers to an experimental study over 30 days, with 30 rats were divided into four groups, the LAB group receiving commercial diet and oral administration of saline, the AIN group that received inducing diet hepatic steatosis and saline administration, CARQMIN group, which received the inducing diet and the trimera baccharis extract in its lower dose (3 mg / day) and CARQMAX group, which received the inducing diet and aqueous extract of baccharis trimera in its highest dose (6 mg / day). Weight gain and food consumption were recorded throughout the trial period, to calculate the coefficient of feed efficiency (CEA). Qualitative phytochemical analysis of aqueous extract of trimera baccharis and serum biochemical tests were performed glucose, lipids and function tests and liver damage, and histological analysis of the liver tissue, to observe the presence and degree of steatosis. CEA increased in all groups that received the diet AIN. There was a significant increase in triglyceride values and VLDL fraction in animal groups treated with the gorse, when compared to the animals of the LAB group. For total and HDL cholesterol, there was a trend towards increase in the animals treated with the coot, especially in their highest dose. Interestingly, the AIN group had AST values lower than those of other groups, and lower ALT that the LAB group. Although there was no difference between groups or the number of cases of hepatic steatosis (P = 0.06), nor in its staging, the set residue frequency of absence of steatosis to the LAB group was 2.8, indicating that This group showed a frequency of no steatosis greater than expected by chance. The use of aqueous extract of trimera baccharis in this study did not prevent or attenuated hepatic steatosis induced by diet; Furthermore, such use made changes in serum lipid profiles of the animals