Perfil metabolômico e farmacológico da Mansoa hirsuta D.C. (Bignoniaceae)

Abstract

Mansoa hirsute (Bignoniaceae), popularly known as Cipo d'álho, is chemically little known, although it is popularly used in the treatment of many disesases. The present studiy aimed to study the biological potential of the extracts and fractions obtained from leaves of M. hirsuta, to isolate and purify the active principles and to study the metabolomics of the plant. Plant materials were collected in five different regions from Semi-Arid in northeastern Brazil and were submitted to classic phytochemistry and analysis of the metabolomics. For that, the brute extract of the plants of Gentio do Ouro was obtained by extraction with ethanol 96% and was submitted to partitioning with different solvents, while the volatile were extracted by Clavenger apparatus. The isolated compounds were identified by GC-MS and NMR. Extract and subfractions were submitted to biological tests to clarify their possible biological activities. The brute extracts of the plants provenientes of the five regions was submitted to NMR analysis RMN to adquire 1D 1H and 2D COSY and J-resolved spectra. NMR data were submitted to chemiometry (HCA, PC and PSL-of the) to classify the species according to their metabolome. Pentacyclic triterpenes ursólico and oleanolic acids, and the steroids stigmasterol, ß-stitosterol and lupeol were isolated, together with dialyl sulfide and trialyl sulfide. The ethyl acetate fraction was able to inhibit the production of NO (57.92%) and proliferation of linfocites (99.92%), while the acid ursólico and oleanolic presented inhibition values greater than 99%. Metabolic analysis allowed to establish that plants of Gentio do Ouro are rich in pentacyclic triterpens, while plants from Boninal contains mostly flavonoids in its chemical composition. The results also suggest that protoantocianidinas are the main constituents of the plants from Caetité, while saponinas are found in the plants from Campo Alegre de Lourdes. Plants of Morro do Chapéu were distinguished form the other regions for being rich in oligosaccharides and aminoacids.