Otimização de extração de gordura em equipamento de soxhlet com múltiplas amostras

Abstract

Food analysis is one of the main points to be observed in the area of animal nutrition, since knowledge of the composition of foods allows the formulation of diets that meet the nutritional needs of foods and contribute to the quality of the feed. The most used techniques for ethereal extraction are: hot method (Goldfish) and cold method (Sohxlet), the latter being the most commonly adopted in animal feed analysis laboratories. The equipment consists of a set for heating, evaporating and condensing the solvent that will “wash” the sample containing the material to be extracted. The present work aimed to optimize the fat ingestion technique with soxhlet equipment to obtain the ether extract (EE) using one to four samples per extractor in order to evaluate and validate the fat ingestion technique using the conventional way with one sample per extractor and comparing with a stepwise increase four samples per extractor for EE engineering in order to improve the analysis time on vitamin intake using one or more samples in the same extractor. Therefore, there was no significant difference (P<0.05) for all four treatments using as selection soybean meal (FS), brewery silage (SC), brachiaria-capiaçu grass (BRS-CA) and root of sweet potato (RBD), making it feasible to optimize the fat inheritance technique with soxhlet equipment to obtain the ether extract (EE) for the inclusion of four samples per soxhlet for the exceptions of soybean meal, brewery silage, silage of BRS-Capiaçu grass and sweet potato root.

Keywords: animal nutrition, ether extract, food analysis, optimization