Atividade antimicrobiana de microencapsulados de própolis vermelha

Abstract

The marketing of products obtained bee (Apis mellifera) as honey and propolis has become common in Brazil, visibly noticeable with the increasing deployment of microindústrias of products based on honey and propolis. The popular use ever-increasing propolis and their derivatives with antimicrobial, anti-inflammatory, antiviral, immunomodulatory and anticarcinogenic have demonstrated the great therapeutic power. As a result, new products containing propolis are being developed, such as the microencapsulated. Depending on the method used, such microencapsulated may result in a final product with negative characteristics. Given the above, this study aimed to evaluate the microbiological profile of the microencapsulated propolis obtained by drying techniques spray-drying and freeze drying. The propolis was obtained from apiaries located in the mangrove area of the municipality of Marechal Deodoro / AL. The sample was collected from February to March 2012. From the extract were prepared 4 formulations varying the amount of ethanol extract of propolis, as well as different proportions of excipients. Two techniques drying (spray drying and freeze drying) were used, resulting in 8 samples of microencapsulated However, these, 4 were selected formulations (each two drying technique) to perform the tests. To evaluate the antimicrobial activity, the determination was made of the content of Inhibitory Concentrations and Minimum Bactericidal (MIC and MBC) through the microdilution technique in broth front of the standard strains of Staphylococcus aureus (ATCC 25922), Escherichia coli (ATCC 25922), and Listeria monocytogenes (ATCC 19115). Biomarkers of propolis were identified using high-performance liquid chromatography (HPLC) coupled to Ultra-Violet detector. The microencapsulated presented antimicrobial activity for all microorganisms studies, but with differences in the CIM. Gram positive bacteria were more susceptible than Gram negative bacteria. The chromatograms obtained by liquid chromatography of high efficiency in crude extracts and formulations propolis base showed a complex chemical composition, with several peaks at different retention times. The microencapsulated tested can be considered candidates for optimization samples propolis base. These results will allow a quality control and, until a future standardization of natural products and nutraceuticals, in order to obtain products with composition of consistency and appropriate nutritional properties.