Efeito hepatoprotetor do alpha-terpineol em ratos espontaneamente hipertensos e infartados por isoproterenol

Abstract

Introduction: The alpha-terpineol (TPN) is a monoterpene obtained from Protium hepthaphylum. That has several pharmacological activities such as: anti-inflammatory, anti-obesity and cardioprotection on Spontaneously Hypertensive Rats (SHR) by isoproterenol model. ISO evoked metabolic disturbance on hepatocytes, stimulate fatty acids storage on vesicles inducing non-alcoholic steatosis. The aims of this study were verify the hepatoprotective effects of alpha-terpineol in SHR against liver damage induced by ISO. Methods: Experimental protocols were approved by the CEUA/UFAL nº09/2015. Male rats were allocated into 5 groups and treated for 15 days (n=5): (G1= saline 0.9% P.O/d); (G2= Infarcted saline 0.9% P.O./d + ISO 85mg/kg 2x s.c); (G3= TPN 25 mg/kg P.O./d); (G4=TPN 50 mg/kg P.O./d) and (G5=TPN 75 mg/kg P.O./d). On 16º day, rats were anesthetized (Ketamine 80 mg/kg + Xylazine 4 mg/kg i.p.) and morphometric tests were performed in accordance with Scherle´s methods. Morphology tests were examined under light by stereoscopy, while lipid levels were estimated by total cholesterol and triglyceride dosages of blood serum. The results were expressed as mean ± SEM, and analyzed statistically by ANOVA one-way followed by Newman- Keuls test considered significant when * p <0.05, ** p<0.001 and *** p< 0.0001. Results:. Induction with ISO in SHR rats induced an increase in liver weight, in addition it also induced an increase in the correlation between liver weight and body weight and an increase in the correlation between liver weight and femur weight. Regarding the morphological and histopathological aspects, it was observed that alpha-terpineol inhibited non-alcoholic hepatic steatosis due to damage caused by induction with isoproterenol, in addition, previous treatment with alpha-terpineol significantly inhibited the increase in cholesterol and triglyceride levels , at all doses tested. In addition, after the glucose tolerance curve, it was noted that there was a reversal in this curve, which may also influence glucose metabolism, however further studies are needed. Conclusion: Taken together, the results indicate that alpha-terpineol has a significant hepatoprotective effect, in hypertensive and infarcted rats.