Efeitos da exposição a um herbicida a base de Metribuzin durante o desenvolvimento inicial do Zebrafish, com ênfase no desequilíbrio redox

Abstract

Metribuzin is a herbicide that belongs to the chemical group of asymmetric triazinones, widely used to control weeds in some crops. Due to its indiscriminate use and leaching, it has been found in groundwater, wastewater and shows persistence in the soil. The accumulation of free radicals due to exposure to contaminants can lead to oxidative stress, suppressing the action of antioxidant enzymes and altering the cellular processes of organisms, causing damage to DNA and biomembranes, physiological changes, teratogenic effects and even cancer. In this study, the potential effects of exposure to a metribuzin-based herbicide during early development of zebrafish (Danio rerio) on redox imbalance were evaluated. Zebrafish embryos/larvae were exposed to different concentrations of the herbicide Sencor 480 - Bayer - (0.0001 mg L-1, 0.001 mg L-1, 0.01 mg L-1) and negative control (reconstituted water) for 144 hours. Next, they were destined to evaluate the activity of the enzymes Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione-S transferase (GST). Parametric data were submitted to ANOVA - ONE WAY followed by Tukey test. The results showed a reduction in the activity of CAT and SOD enzymes, indicating an increase in reactive species and a decrease in antioxidant capacity. The levels of the GST enzyme were reduced in all tested concentrations, indicating an overload of reactive species, making its detoxification difficult. These data indicate that exposure to the herbicide based on Metribuzin induces a redox imbalance and promotes oxidative stress, corroborating previous studies in fish and other animal models, calling attention to the risks to animal health associated with the indiscriminate use of this pesticide.