Estudo estrutural e avaliação da citotoxicidade de uma hidroxiapatita dopada com Silício preparados por reação mecano-química

Abstract

In this work we studied powders Hydroxyapatite (Hap) and hydroxyapatite doped silicon (HapSiX, X = 0.0, 0.1, 0.2, 0.5, 0.7, 1.0) at different concentrations prepared by Mechano-Chemical reaction (RMQ) using alternate as a precursorfor the synthesis of the eggshell of the chicken by its high content of calcium carbonate. After addition, the obtained powders were heated separately at 600 °, 700 ° and 900°C for 6 hours for each sample. The structural study was performed with the technique of X-Ray Diffraction (XRD)and Rietveld Method (RM), the morphological analyzes were performed with Scanning Electron Microscopy (SEM), EDX and Chemical Mapping (MPQ), moreover, made it biological studies is to evaluate the cytotoxicity of the samples prepared. XRD results showed the presence of single phase associated withhydroxyapatite to HapSiX = 0.5, were also found for other strengths phases associated with Tricalcium Phosphate, calcium oxide, calcium silicates and quartz. The results showed changes in the MR parameter of the network due to the dopant material, and a large crystal size decreased with increasing dopant material. The results obtained by SEM, EDX and MPQ present a good porosity as well as a great homogeneity in the samples of Hap and HapSi for all concentrations. The present test a cytotoxic cell proliferation for the large samples in comparison with the silicon without Silicon also observed differences related to the size of the powders.