Efeito cardioprotetor da fração de acetato de etila do extrato de Trichilia catigua em ratos infartados

Abstract

Introduction: Trichilia catigua is a tree distributed in the Atlantic Forest known as "catuaba" that is used by the population as a tonic for its attributed aphrodisiac and memory stimulating functions. Studies confirm that T. catigua has antioxidant activity, acting in the central nervous system stimulating hippocampal proliferation, cognition, protector of cerebral infarction, antinociceptive and antidepressive activity. Phenolic compounds are the main components of T. catigua bark and are commonly used for medicinal purposes. Objective: To evaluate the cardioprotective potential of ethyl acetate fraction of T. catigua extract (AETC) in infarcted rats. Method: Male Wistar rats were randomized into groups (n = 5) AETC treated for 30 days and the induced infarct with isoproterenol. After euthanasia, blood and tissue were used for analysis of morphometric parameters and biochemistry. Approaches have been used in silico, in vitro and in vivo in order to identify courses of action involved in the cardioprotective effect. Results: Expressed as mean ± SEM and analysis of variance One-way ANOVA followed by multiple comparison test Newman-Keuls, p <0.05. AETC decreased (p <0.001) isoproterenol (ISO) induced cardiac hypertrophy in the variables heart weight, left ventricular weight, left ventricular thickness, mean area and volume of cardiomyocytes. And decreased non-dose dependent, ISO induced cardiac necrosis in the creatine kinase MB fraction, total creatine kinase, lactate dehydrogenase, glutamic oxalacetic transaminase, área of necrosis after staining with triphenyltetrazolium chloride, volume density, profile density, number density and number of intact cardiomyocytes. In addition, AETC showed a significant hypotensive, bradycardic, vasorelaxant and antioxidant effect. Conclusion: Chronic treatment with AETC cardioprotective effect for myocardial infarction in rats by altering variables related to hypertrophy and cardiac necrosis caused by isoproterenol.