Estudo metabolômico do filtrado da cultura do fungo endofítico Rhizoctonia solani

Abstract

Fungi constitute a microorganism group that has great scientific and practical interest. Some species of fungi have been used in the production of foods, beverages and medicines. Being the Rhizoctonia solani, one of the few fungi found in almost all continents and having poles plants as host, your metabolism tends to undergo some changes due to interaction with the host plant and regional variations. In a Canadian study, it is described as a pathogen, it was isolated cyclic dipeptides of type 2.6-dioxopiperazinas of the pathogen Rhizoctonia solani culture, already in a Brazilian study, with the endophyte Rhizoctonia solani, of the plant S. terebintifolius Raddi, popularly known as aroeira, it was reported that the methanol extract of mycelium was obtained antinociceptive activity in rats. With the objective of studying the metabolism present in culture filtrates (CF) of endophytic Rhizoctonia solani, the fungus was grown in six Erlenmeyer flasks containing 100 mL of the BD medium for eight weeks, without agitation, in the dark. Every 7 days, 1.5 mL aliquots of medium, were filtered in Millipore® (0.22 μM). Each fraction of 300 μL of these were added for 300 μL of phosphate buffer solution (pH 7.4, containing 1 mM of TSP), 50 μL of D2O and transferred to own tubes, and analyzed by 1H-NMR (400 MHz Bruker, UltraShild). Data were preprocessed using the TopSpin® program, and the region containing the sign of the water was suppresses using NOESY1D experiment. For metabolites identification it was used literatures databases and of the Chenomx® program, verifying the presence of acetaldehyde, acetate, alanine, betaine, citrate, ethanol, methanol, succinate and uracil. The data were then standardized and aligned before being subjected to multivariate analysis using the MatLab® program through tools developed by Dr. K. Veselkov (Imperial College, London, UK) and SIMCA®, although secondary metabolites are not detected in the culture filtrate, it was observed high yields of ethanol.