Associação dos polimorfismos da IL6 T15A (rs13306435) e iNOS -1173 C>T (rs9282799) com a susceptibilidade a infecção e progressão da dengue

Abstract

Dengue is a public health problem with millions of cases reported worldwide. The infection with dengue virus has a variety of signs and symptoms including asymptomatic and symptomatic cases. The immune system is involved in the host's response to infections as well as in the pathogenesis of diseases, including dengue. Cytokines and nitric oxide are componentes of the immune system. Interleukin 6 (IL6) is a cytokine that plays an important role in linking the innate immune response to the acquired immune response. Inducible nitric oxide synthase (iNOS) is an enzyme responsible for inhibiting viral replication through the production of nitric oxide. Genetic polymorphisms may influence gene regulation and protein structure and function. The study aimed to investigate the relationship of polymorphisms in iNOS (-1173 C / T) and IL6 (T15A) genes with dengue infection and disease progression. It was a case-control study with patients infected with dengue virus and healthy controls of the population. The genotypes of the iNOS (-1173 C / T) and IL6 (T15A) genes were determined by the real-time polymerase chain reaction (qPCR), using the allelic discrimination method. The analyzes of the correlations between genotypic and allele frequencies concerning susceptibility to infection were obtained using the BioEstat 5.3 software. p-value less than 0.05 were considered statistically significant. The study was composed of 179 individuals, 27 of the dengue fever group, 33 of the dengue hemorrhagic fever group, and 119 of the control group. The T15A polymorphism of the IL6 gene, showed no association in the allelic and genotypic distribution between the groups. The -1173 polymorphism of the iNOS gene, showed no association in the allelic and genotypic distribution between the groups. No association was found between the polymorphism of IL6 T15A and iNOS -1173C/T with dengue infection.