Padronização do cultivo de plântulas de tomate (Solanum lycopersicum L.) em laboratório para aplicação em teste ecotoxicológico

Abstract

Nowadays, tomatoes are one of the best known and most consumed vegetables in the world and are grown in tropical, subtropical and temperate regions. Due to the growing importance for agriculture and commercial interest, tomato germination / growth has been studied. Several internal and external factors can interfere with seed germination and seedling growth, such as the light intensity that can interfere positively or negatively. The objective of this work is to standardize a methodology for effluent ecotoxicity evaluation using tomato seeds (Solanum lycopersicum L.; Solanaceae), being four different cultivars (Carolina, Yashi, Kada and Chapolin), observing the development of irrigated seedlings with effluents, optimizing operational parameters. The main parameters evaluated will be light intensity, daily amount of water in the irrigation process and substrate (soil) / plant ratio in the crop. The variables analyzed will be the percentage of seed germination, shoot and root length produced by each cultivar. With the best values obtained in the standardization, ecotoxicological tests will be performed with chemical and agroindustrial effluents, specifically malachite green and whey, comparing them with the control results, in order to validate standardization achieved in the development of this work.