Uso de semioquimicos na interação trófica no manejo de pragas

Abstract

This studes entitled "Use of semiochemicals in trophic interaction in pest management " describes the detailed analysis of the interactions between these two pests and cucurbit plants with the aim of developing more effective management strategies. Below, a summary of each chapter that makes up this research is presented. This thesis comprises seven chapters. The first chapter establishes the context of the research, highlighting the economic and agricultural importance of cucurbit crops worldwide. It also presents the main pests studied, such as Diaphania hyalinata and Liriomyza sativae, describing the negative impacts on cucurbit crops. Chapter 2 deals with the collection and analysis of cuticular hydrocarbons (CHCs) from Liriomyza sativae and volatile organic compounds (VOCs) from melon (Cucumis melo) and jack bean (Canavalia ensiformis) plants, highlighting the difference in cuticular hydrocarbon profiles between male and female insects. Volatile compounds, including aldehydes and ketones, essential for communication between insects and their host plants, were identified and tested in olfactometry bioassays. In Chapter 3, we analyzed the chromatographic profile of VOCs from male and female insects of the moth D. hyalinata, showing that females released a greater number and quantity of VOCs compared to males. In addition, the compound (Z, Z)- 10,12-Hexadecadienal, previously reported as a component of the pheromone and present in VOCs collected from female insects, was identified. Chapter 4 reports the study of annatto seeds (Bixa orellana) and their potential use in developing pest control products. Different extraction techniques and solvents are examined, and their efficacy in obtaining active compounds such as Geranylgeraniol is compared. Chapter 5 analyses a set of VOC data collected from different cowpea (Vigna unguiculata) genotypes. The experimental design and methodology used for collecting and analysing VOCs are discussed, highlighting the importance of these data for understanding the variation of VOCs emitted by cowpea genotypes. Chapter 6: The use of cis-jasmone as an inducer of resistance in cowpea plants against the black aphid (Aphis craccivora) is evaluated, including studies on the collection of volatile compounds and the effects on the biology of the aphid. Chapter 7. The last chapter provides a comprehensive review of insect pheromones that affect stored grains, discussing current findings and their implications for managing these pests.