Bioespectroscopia de células vermelhas em dispositivo de acustofluídica

Abstract

Hemopathies are considered blood disorders capable of altering the health of the affected patient, they can be hereditary or acquired throughout life, arising in the blood components themselves in the processes of formation of red cells. The most common blood disorders in Brazil are anemia, blood disorders, blood clots and finally blood cancer, with early diagnosis being a determining factor for treatment. However, access to diagnosis and hematological exams is linked to the economic condition of the patient, therefore, clinical treatment is started in more severe stages of the disease. This work aims to study and develop new technologies with minimally invasive diagnostic capability based on Raman vibrational spectroscopy in red cells for the identification and differentiation of possible hemopathies at lower costs. The platform uses Raman optical spectroscopy methods combined with an acustofluidics device capable of trapping and levitating particles through the force of acoustic radiation, thus enabling the investigation of biochemical properties of a separate cell or a cluster of cells without contact with walls or of the submillimeter cavity substrate. Through the optoacustofluidics platform, we obtained with a 532nm laser from a set of normal cells and heated red cells, the Raman spectra. Then, we applied the principal component analysis method in a matrix of 32 samples by 513 spectral lines in order to distinguish the groups, as the difference in intensity corresponding to the average spectral bands provide an indication of the biomolecular components that underwent alteration with the heating process.