Aplicações tecnológicas de moléculas bioativas obtidas a partir de resíduos do beneficiamento do camarão sete-barbas (Xiphopenaeus kroyeri)

Abstract

Sustainability in supply chains tied to fishing has become one of the biggest challenges for the fishing industry, especially regarding the management of fish processing waste, byproducts and accompanying fauna. Heads, exoskeleton, paws and tail are the main residues that result from processing of shrimps, these subunits can perform the extraction of chitin and later conversion into a biopolymer chitosan that is extremely broad biotechnological application because of the versatility of chitosan molecule. Its application in processes for immobilization of proteins has advantages when compared to the action of free proteins, including possibilities for application in the detection of anti-nutritional factors that are present in many dietary components used in aquaculture. In this study intestines of Nile tilapia were homogenized with 0.05M phosphate buffer to obtain proteases that were immobilized on a magnetic support chitosan. The purified trypsin had approximate molecular weight of 23 kDa. The optimal time for immobilization of trypsin in the holder was 8 hours. Greater catalytic activity was recorded at 50 ° C (1,101 ± 0.0371 mμ / mg). When performing the verification of dietary components that m had greater inhibition of catalytic activity of purified trypsin, at was observed a greater inhibition in feed with 25% crude protein. The tests feasibility of immobilized enzyme were positive by over 60 days of the immobilized enzyme, these results shows that are good possibilities to use the immobilized proteases on chitosan magnetic bioprocesses.