Atividades proteolíticas da peçonha e do muco tegumentar do Thalassophryne nattereri

Abstract

The Thalassophryne nattereri is a venomous fish, small, inhabiting almost all Brazilian coast. It is relatively frequent accidents among workers fishery in the North and Northeast regions of Brazil, and also from sunbathers cause. Its venom causes an intense inflammatory reaction, followed by necrosis of the affected area, which persists for several days. How often the venoms in T. nattereri is a serous and rich in protein secretion . Among these are various peptides and proteases, which have complex effects on the human body. In this dissertation, we seek to investigate these proteolytic activities, not only but also from the venom of the cutaneous mucus from T. nattereri, in relation to collagenolytic activity and activity to trypsin/chymotrypsin/elastase-II like. The venom showed no collagenolytic activity. None of the preparations demonstrated to activity like trypsin/chymotrypsin/elastase-II against BApNA chromogenic substrates, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide and N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide. We also assessed the proteolytic properties of cutaneous mucus from T. nattereri. This has collagenolytic activity, with at least 5 proteins bands capable of degrading collagen. By having collagenolytic activity and there is the possibility that it is neutral endopeptidase such as metalloproteinases or the case of two different enzymes, such as carboxypeptidase and a metalloproteinase. In the future, the isolation of these enzymes active against substrates in the venom and cutaneous mucus from T. nattereri will be needed to better characterize and identify their properties and possible applications.