Plastoma de Syagrus coronata (Martius) Beccari e análise filogenética em Arecoideae (Arecaceae)

Abstract

Syagrus coronata classified as belonging to the family Arecaceae, subfamily Arecoideae, is a species of palm native to Brazil that has great economic, ecological and social importance. The genus Syagrus has 59 species, and its origin is dated to about 27.02 million years and has Cocos as its closest genus. The objective of the work was to assemble the plastidial genome of S. coronata using NGS data and perform a phylogeny using complete chloroplast genomes for the subfamily Arecoideae. A total of 77 million Illumina single end reads of 100 bp were mapped to obtain the S. coronata genome. The complete genome of S. coronata and other Arecoideae genomes available in NCBI were used to obtain the phylogeny of Arecoideae. The genome of S. coronata showed 155,053 bp, with IRA having 26,476 bp, IRB 26,647 bp, LSC with 84,410 bp e SSC with 17,520 bp. We identified 90 genes, 8 rRNAs, 39 tRNAs and 30 repetitive regions. The comparison of the plastidial genome of S. coronate with the Arecoideae species showed Cocos nucifera as the closest and Chamaedorea seifrziii as more divergent, and phylogenetic analysis using the plastid genomes corroborates with previous phylogenies for the subfamily Arecoideae. It is concluded that the use of Illumina single end reads was efficient to sequence the plastidial genome of S. coronata, and that phylogeny using complete chloroplast genomes presents similar topology when compared to phylogeny using few plastid regions and nuclear genes.