Ação conjunta do fator-1 de crescimento semelhante à insulina (IGF-1) e da quimiocina CXCL12 na migração de linfócitos

Abstract

Insulin-like growth factor-1 and CXCL12 chemokine are produced by immune cells and several studies have been published about their effects on leukocytes. Aim of this study was evaluate combined effect of insulin-like growth factor-1 (IGF-1) and CXCL12 chemokine on lymphocyte migration in vitro. Fresh lymphocytes were obtained from subcutaneous lymph nodes of C57BL/6 mice, and their subsets (B cells and CD4+ and CD8+ T cells) expressed diverse membrane receptors, as IGF-1R, CXCR4, CD62L, VLA-4 and VLA-5. IGF-1+CXCL12 treatment increased VLA-5+ and CD62L+ B cells. However, CXCL12 treatment decreased VLA-5+ B cells. Furthermore, in cell adhesion assay, IGF-1 promoted a decline in the number of lymphocytes (all subsets) adhered to endothelial cells, although VLA-4 expression was unaltered. In cell migration assay, IGF-1 and CXCL12, separately or jointly, improved rates of migrating lymphocytes (all subsets), applying fibronectin coating or not. Interestingly, in CXCL12 group, CD62L receptor was augmented on migrating cells and this chemokine blocked IGF-1R transcription. This results indicate that IGF-1 and/or CXCL12, combined to fibronectin, enhance B and T cell migration in vitro.