Avaliação do efeito renoprotetor induzido por alfa- terpineol em ratos SHR e infartados

Abstract

Introduction: α-terpineol (TPN) is a monoterpene obtained from the resin of the stem of Protium heptaphyllum. This compound has pharmacological activities, such as antiinflammatory, vasorelaxant and cardioprotective activities in infarcted and hypertensive rats, cardioprotective activity was also observed. Isoproterenol (ISO) induces disturbances and alterations in the cardiovascular system, as well as in target organs such as the kidneys. The aim of this study was to verify the renoprotective effects of TPN in infarcted and hypertensive rats against ISO-induced renal damage. Methodology: The experimental protocols were approved by CEUA / UFAL nº 09/2015. Male rats were divided into 5 groups and treated for 15 days (n = 5): (G1 = saline 0.9% V.O./d); (G2 = infarcted saline 0.9% V.O./d + ISO 85 mg/kg 2x s.c); (G3 = TPN 25 mg/kg V.O./d); (G4 = TPN 50 mg/kg V.O./d) AND (G5 = TPN 75 mg/kg V.O./d). On the 16th day, the rats were anesthetized (Ketamine 80 mg/kg + Xylazine 4 mg/kg i.p.) and morphometric tests were performed according to Scherle's methods. Morphological tests were examined under light by stereoscopy, while creatinine and urea levels were estimated by blood serum dosages. The results were expressed as mean ± SEM and statistically analyzed by one-way ANOVA followed by the Newman-Keuls post test, considered significant when * p <0.05, ** p <0.001 and *** p <0.0001. Results: Right kidney weight (G1=1.062 ± 0.01/g**** vs. G2= 0.456 ± 0.08/g) and left kidney weight (G1= 0.456 ± 0.08/g vs. G2 = 0.464 ± 0.06 /g), right kidney weight to body weight ratio (G1 = 0.355 ± 0.010**** g/g* vs. G2 = 0.176 ± 0.030 g/g), (G3 = 0.209 ± 0.01**g/g* vs. G2= 0.176 ± 0.030 g/g), (G4= 0.203 ± 0.02**g/g* vs. G2= 0.176 ± 0.030 g/g) and (G5= 0.289 ± 0.01** vs. G2 = 0.176 ± 0.030 g/g), ratio of left kidney weight to body weight (G1 = 0.351 ± 0.016**** g/g* vs. G2 = 0.179 ± 0.010g /g), (G3= 0.214 ± 0.02*****g/g* vs. G2= 0.179 ± 0.010g/g), (G4= 0.205 ± 0.02*****g/g* vs. G2 = 0.179 ± 0.010g/g) and (G5 = 0.300 ± 0.01*** vs. G2 = 0.179 ± 0.010g/g). Through the morphological aspects, it was observed that the TPN inhibited the renal injury induced by ISO, in addition, the previous treatment with TPN significantly inhibited the increase of the serum creatinine levels (G1= 2,500 ± 1,400**** vs. G2 = 14.200 ± 3.200), (G3= 2.900 ± 1.20*** vs. G2= 14.200 ± 3.200), (G4= 01.100 ± 0.20**** vs. G2= 014.200 ± 3.200) and (G5= 0.500 ± 0.10**** vs. G2 = 14.200 ± 3.200) and serum urea (G1 = 49.800 ± 7.100**** vs. G2 = 504,000 ± 76.700), (G3 = 33.300 ± 10.00***) *vs. G2= 504,000 ± 76.700), (G4= 30.600 ± 9.30**** vs. G2= 504,000 ± 76.700) and (G525.900 ± 8.30**** vs. G2= 504,000 ± 76.700) ). Conclusion: Taken together, the results suggest that TPN induced a renoprotective effect, however, further studies are needed to elucidate the mechanisms underlying this response.