Produção e purificação da(s) celulase(s) produzida(s) pelo fungo Coleosporium plumeriae em diferentes meios de cultura

Abstract

The countless discussions regarding the importance of sustainability in productive activities and concerns about the problems related to the generation of agro-industrial residues have been highlighted, since it becomes increasingly necessary to adopt alternatives to replace the use of fuel fossils. Thus, lignocellulosic materials, the most abundant bio-renewable resource in nature, with low cost, mainly composed of cellulose, hemicellulose and lignin, present themselves as a promising alternative in obtaining fermentable sugars for the production of biofuels and macromolecules, such as hydrolytic enzymes. In this context, the present study aimed to produce, isolate and identify cellulases produced by Coleosporium plumeriae. This filamentous fungus was isolated on potato-dextrose-agar culture medium from the spores. The enzymatic production profile of cellulases by solid state fermentation (FES) was evaluated in different culture media, sugarcane bagasse, wheat bran and wood powder in a bacteriological oven at a temperature of 27.5ºC. From the wheat bran medium, the medium with the highest enzyme production, 220.76 U/g, a crude extract was produced to begin cellulase purification. The enzyme was completely isolated by a precipitation using ethanol, having its highest recovery in the 80-100% ethanol fraction and followed by a size exclusion chromatography. The purification of cellulase was confirmed using a 12% silver-stained polyacrylamide gel, which shows a single band in chromatographic fractions 18 and 20. The study demonstrated the feasibility of producing cellulase using low-cost media (from agro-industrial residues) and the unprecedented isolation of a cellulase produced by Coleosporium plumeriae.