Incidência e caracterização molecular de badnavírus em tropicais no estado de Alagoas

Abstract

One of the most promising segments of national agribusiness is floriculture, with an emerging profitable market all over the world. Tropical flowers are attacked by different pathogens, including badnaviruses. Badnaviruses (Family Caulimoviridae) consist of a group of viruses with a double-stranded circular DNA genome, encapsidated in non-enveloped bacilliform particles and transmitted mainly by mealybugs. Diseases caused by viruses of this genus have emerged as serious problems for many important economic crops in the tropical and subtropical regions of the world, including Brazil. For the influence of the incidence of badnavirus species that occur in tropical plants of the Costaceae, Heliconiaceae and Zingiberaceae families, the sample was collected in the poles producing tropical plants in Alagoas. Subjected to detection of badnavirus, via PCR (“Polymerase Chain Reaction”) using universal primers for the genus. Fourteen sample Costus spiralis (8), Alpinia purpurata (4) and Helicônia sp.(2) were positive, later the analyzes were confirmed to the amplification of the complete viral genome via Rolling circle amplification (RCA) using the TempliphiTM 100 amplification kit (GE Healthcare Life Sciences) and again we continued PCR to detect the occurrence of badnavirus infection in the episomal form. After identifying a sample infected with the virus in the episomal form, the RCA product was sent for high-throughput sequencing. The complete viral genome sequence was collected and observed by BLASTn analysis, pairwise analysis in the SDT program for species demarcation, and RPD for identification of recombination events. A new isolate of Badnavirus alphavirgamusae (BSGFV) was identified, in this work referred to as BSGFV_BR_ALPINIA.

Keywords: Caulimoviridae, Zingiberales, high-throughput sequencing.