Caracterização morfocultural e molecular de isolados de Colletotrichum agente causal da antracnose do Feijão-Fava no estado de Alagoas

Abstract

Twenty monoconidial cultures of Colletotrichum isolated from leaves of lima bean (Phaseolus lunatus L.), were characterized morphology by conidial and appressoria , colony color, presence of setae, growth rate of colony, sensitivities to Maxim, PCR with taxon Colleotrichum-specific primers and phylogenetic analysis. Twenty isolates were identified as Colletotrichum truncatum based on morphology of conidia and appressoria, and most isolated way of conidia and appressoria globose type falcado, clavate and / or oval. Presence of setae, color of colony, sensitivities to Maxim, growth rate were variable among isolates and influenced by the culture medium. All isolates were pathogenic to lima bean and applied by the leaf highlighted method. PCR with specific primers confirmed the identity of 11 C. truncatum. Genetic characterization with Primmer ITS 1 and ITS 4 were effective in molecular identification of 60% of the isolates of C. truncatum. Thus, studies using other molecular markers are needed to make further analysis of genetic variability among isolates of this species of fungus.