Associação de marcadores moleculares microssatélites com o teor de fibra em cana-de-açúcar

Abstract

The cane sugar has a high potential for biomass production. Part of this energy source is in the form of sucrose, used to produce sugar and ethanol. In this process, the waste or residue generated (consisting of fiber), are used for the cogeneration of electricity, where Brazil has a total of 2,529 enterprises in operation, generating 116,611,624 kW / year which is scheduled for the next year an addition of 46,987,830 kW in generating capacity in the country, from the 137 projects currently under construction and more than 504 granted (UDOP, 2011). The current varieties of cane sugar present a variation to the fiber, it is possible to develop varieties with higher levels of fiber, without the occurrence of large losses in the amount of sucrose, which could compromise the production of sugar. Therefore, it is essential that there is genetic variability, allowing for selection of superior genotypes for the trait in question. The objective of this study was to develop a system of type SSR markers associated with fiber content and analyze the genetic variability of sugar cane genotypes constiuintes the germplasm bank of Sierra Gold, Murici, Alagoas, Brazil. We analyzed 15 accessions of cane sugar with 22 SSR primers. The results showed a number of alleles ranging from 3-12 per individual, with average peak between the primers of 0.794, minimum 0.434 (SCC92) and maximum 0.951 (SCC76). The discriminatory power of the primers showed an average of 0.779, which showed a SCC76 (DJ) up to 0.947. The likelihood of confusion ranged from 0.052 to 0.607 in SCC76 and SCC92 primers respectively, with average of 0.220, with an "r" of 0.47 which suggests a high correlation using primers SCC72 and SCC92. The use of SSR was efficient to determine access to genetic profile analyzed and their correlation with the characteristics analyzed, can be used as a system for obtaining new varieties with traits of interest such as the fiber.