Obtenção e caracterização de complexos de inclusão com extrato de própolis marrom de União dos Palmares -AL

Abstract

The first records of the use of propolis by man date back to ancient Egypt. In Brazil, its use is used in popular medicine as a complementary therapy in the treatment of various diseases. Propolis is a resinous material made by A. meliferas bees that collect the raw material from various plant sources, transforming them by adding salivary secretions and wax. It is highly variable in terms of physical aspects, depending on the botanical and geographic origin and time of its collection. The most popular technique for producing propolis extracts is extraction with ethanol, however it has disadvantages and limitations for application in industry. Therefore, to overcome this problem, switching to aqueous suspensions is an option, but biologically active substances have low solubility in water. Therefore, the development of inclusion complexes (IC) of β-cyclodextrins (β-CD) and brown propolis extract from União dos Palmares (EPMUP) is a solution to this low solubility. One of the most used methods to improve the stability and water solubility of polyphenolic compounds is the preparation of IC with cyclodextrins (CD). There are several methods for producing CD ICs, the method chosen for the β-CD:EPMUP complex was aggregate coprecipitation with the freeze-drying method as a drying method, physical mixtures (MF) were obtained for comparative studies. To prove the formation of IC as well as which one was more efficient, analysis methods such as thermogravimetry (TG), X-ray diffractometry (XRD), determination of total flavonoids, antioxidant activity, in vitro dissolution tests and microscopy were used. scanning electronics (SEM). With the results obtained in DRX and in vitro dissolution, the formation of the CI EPMUP:β-CD was confirmed, in DRX the disappearance of crystalline peaks characteristic of β-CD was observed, indicating a reordering of the crystalline structure of the CI, the results obtained by in vitro dissolution were a dissolution peak of 32 μg/ml for IC 1:2, with a possible total of 50μg/ml, where if around 64% of all IC was dissolved, the formation of IC generates an increase in solubility in an aqueous medium of the organic compounds present in EPMUP. In this scenario, a great option is the development of formulations and products, which cannot contain alcohol, using the CI EPMUP:β-CD