Avaliação do risco de infecção cruzada através das linhas d’água dos equipos odontológicos na rede pública estadual de Alagoas

Abstract

During routine work, the dental staff and patients are exposed to contamination by water used in dental units, through accidental ingestion or aspiration of aerosols generated by high-speed and air-water syringe. This study evaluated the water quality of the dental equipment of public dental offices in Alagoas, conducting the count of heterotrophic bacteria, filamentous fungi, as well as the presence of total and fecal coliforms and Streptococcus mutans. We collected water from air-water syringe, supply hoses with handpieces disconnected, handpieces with supply hose attached, reservoir and water where the reservoir was filled from six dental offices. To search for heterotrophic bacteria and fungi volumes of 100 μL of pure sample and 10-1 dilution were plated in duplicate on plate count agar medium supplemented with 50 mg.L-1 of Ketoconazole and Sabouraud dextrose agar supplemented with chloramphenicol and amplicilina, 50 mg.L-1 of each. The results were expressed in CFU/mL. For quantification of total and fecal coliforms, we used the method of enzyme substrate (Colilert ®) and the results were expressed in MPN/100ml, according to a table supplied by the manufacturer. The research of S. mutans was accomplished through the use Mitis Salivarius Bacitracin with and without prior disinfection with alcohol 70% of the collection points in Petri dishes and a vial with medium Mitis Salivarius Bacitracin Sucrose without prior disinfection of the collection points. All results were expressed in CFU/mL and subjected to molecular analysis to confirm the identities. We did not observe the isolation of Streptococcus mutans. However the high contamination found indicated that the water was unfit for human consumption can be considered a potential source of cross infections