Desenvolvimento de materiais odontológicos de alto valor agregado contendo própolis vermelha de Alagoas

Abstract

This thesis addresses alternative treatment for tooth caries, one of the endemic diseases that affects all humanity. It also presents the use of a natural product from regional biodiversity – Brazilian Red Propolis (PVA), an exclusive bioproduct from Alagoas, Brazil - as an additive for dental supplies that improves the quality of caries treatment, adding value and increasing treatment efficiency. The PVA obtained from a controlled source was prepared to obtain the hexane extract, which was duly qualified by liquid chromatography to determine flavonoid and phenol contents, the results of which reached between 7.35% of the flavonoid and 117.6 mg of total phenols per gram of dry hexane extract. A DPPH scavenging study was carried out, where the IC50 of the DPPH radical scavenging capacity of the PVA hexane extract was 9.22 μg/mL while the IC50 of Trolox, used as standard, was 7.02 μg/mL. After qualifying the extract, 5 different ionomer compositions were formulated with the addition of PVA dry extract, which were subjected to antibacterial activity tests by diffusion, which confirmed the ability to release daidzein, liquiritigenin, pinobanksin, isoliquiritigenin and formononetin. Mechanical surface roughness tests were carried out, which revealed similar values between the surfaces of the glass ionomer in the control group and that of the ionomer with 0.5% hexane extract. The analysis of the microhardness of materials (Knoop hardness) proved that there was statistically significant difference in the microhardness of the additives in relation to the commercial product of the non-additivated ionomer. The determination of the (qualitative) fluoride release profile was carried out by NMR using an unprecedented procedure, revealing the viability of fluoride release, regardless of the presence of PVA in the ionomer mixture. A fluoride release study was also carried out using fluorometry, demonstrating the quantitative release of fluoride, both in the presence and absence of PVA. The results of biological, chemical and mechanical tests, carried out in vitro and here presented, are very favorable to the indication of the use of PVA as an additive in glass ionomer, allowing us to recommend moving towards in vivo studies.