Produção, isolamento parcial e caracterização de lacases, caseinases e colagenases de Amphobotrys ricini obtidas por fermentação em estado sólido

Abstract

Introduction: Amphobotrys ricini is a phytopathogenic fungus that infects a plant species known as castor bean. It is known that the fungus produces enzymes of biotechnological importance to colonize plants. Given the little knowledge about its strengthening products, it is important to investigate its ability to produce proteases and ligninases. Objective: To produce, partially isolate and characterize proteases and ligninases produced by A. ricini through solid state cultivation and propose applications for the transmitted enzymes. Methodology: The fungus was cultivated on different residues and agro-industrial co-products, to define the best conditions for the production of proteases and ligninases, and from them, the enzymes were extracted and provided for isolation and characterization techniques. The external lignins had their dye discoloration potential measured and the proteases were maintained for activity tests on casein, collagen and milk coagulation. Results and Discussion: Of the investigated ligninases, only laccases were found. The fungus was able to promote discoloration in a culture medium containing bromophenol blue. The laccases were partially separated by salt precipitation and ion exchange chromatography. Among the activities developed by the proteases, it was possible to confirm the activity on casein, collagen and the ability to coagulate milk. Enzymes were partially purified by ingestion in a biphasic aqueous system. Conclusions: The fungus is a producer of laccases capable of bioremediation of dyes and other contaminants from industrial effluents, its caseinases can be applied in the manufacture of dairy products and the presence of collagenolytic activity was confirmed, which is of great importance in the food and pharmaceutical industry.