Bioprospecção de bactérias lignocelulolíticas a partir do intestino de larvas da Helicoverpa armigera (HÜBNER) (Lepidoptera: Noctuidae)

Abstract

Lignocellulosic materials have as main components lignin, hemicellulose and cellulose. It present in the innumerable sources of biomass and due to their abundance and diverse purposes make it materials with high added value mainly by to make viable together the microorganisms that produce cellulolytic enzymes the biological process of production of 2G ethanol among other products. In the present work, microorganisms present in the intestine of Helicoverpa armigera larvae (HÜBNER) (LEPIDOPTERA: NOCTUIDAE) degrade Forage Palm (Nopalea cochenillifera) were used as a source of biomass objectifying to isolate and identification of microorganisms used in the production of 2G ethanol. By means of the existing interaction of Congo red dye with polysaccharides that have β-1,4-D-glucopyranosyl their respective cellulase producers are rapidly and qualitatively determined, this interaction with the dye produces a halo indicating a hydrolysis And to provide the enzymatic index that allowed to determine a catalytic activity of cellulases and the activity of extracelular CMCase. From the collected larvae (N = 15), only nine (09) of them were fed on the forage palm and only three (03) of them presented bacterial growth. Two (02) bacterial isolates were obtained where only one (01) showed degradation of the lignocellulosic material as carbono source. The present study used as substrate in the determination of the catalytic activity of cellulases sugarcane bagasse, forage palm meal and soybean meal, presenting 4,79, 6,72 and 8,69 as enzymatic index, respectively. In the extracellular CMCase activity CMC was used as supplement, presenting 4.58 enzymatic index. Different substrates were used in the determination of endocellulolytic enzymatic activity, using CMC (4.38 UI.mL-1), celulose (UI.mL-1) and sugarcane bagasse (4.17 UI.mL-1). Optical density allowed to plot a maximum growth curve of the bacterial isolate in a liquid culture, which over 32 hours of incubation showed peak growth at 23 hours. The extraction and purification process of the DNA obtained material with a reference band at 450 Bp of the material.