Identificação botânica e caracterização molecular de espécies de erva-de-passarinho presentes no estado de Alagoas

Abstract

The botanical families Loranthaceae and Viscaceae consist of chlorophyll shrubs generally mistletoes trees and shrubs, removing their hosts water and minerals through their roots modified haustoria calls. Given the above, this study aimed to identify species of parasitic plants in citrus orchards and pine cone. Initially we were visited citrus-producing areas and pine cone with parasitism history. They collected three branches (fertile and infertile) of each mistletoe, being labeled plant material, georeferenced and herborized for botanical identification. Total DNA was extracted from leaf samples and used in PCR with primers 1 and 4. ITS amplicons were sent for sequencing in Macrogen Inc. (Seoul, South Korea). The sequences obtained were initially analyzed using the BLASTn algorithm and the non-redundant nucleotide database GenBank mistletoes to determine the species with which they share greater sequence identity. Which were used for inference of phylogenetic trees. They were found four kinds of herb-of-finch, Passovia, Struthanthus, Phoradendron and Psittacanthus belonging to two botanical families (Loranthaceae and Viscaceae), totaling eight different species, parasitizing different hosts. BLASTn and phylogenetic analysis showed that only one of the species retained here had nucleotide sequences deposited in GenBank database. Moreover, these are the first reports of Passovia pyrifolia (Kunth) Tiegh. and Struthanthus marginatus (DESR.) G. infecting orchards of Citrus sinensis in the state of Alagoas and Phoradendron mucronatum (DC) Krug. &. Urb. And Phoradendron quadrangulare (Kunth) Griseb. parasitizing Annona squamosa L. in Brazil.