Obtenção e caracterização de extratos galênicos e extratos secos spray-dryer da própolis vermelha de Alagoas

Abstract

The nutraceutical products based propolis has gained worldwide market due to its excellent antioxidant, antimicrobial, tissue healing, inflammatory, anticancer properties. The red propolis from the Alagoas, a province from northeasten of the Brazil, is proven antimicrobial, tissue regeneration and antioxidant activities. To solve some technological problems in the production of nutraceuticals based on propolis, poor handling of raw materials due to resinous characteristics and the need to stabilize the intermediate product due to its photostability, there was a need for development of analytical tool for quality control using HPLC method and dissolution method to demonstrate the releasing of dried extracts of red propolis. The dried extracts A, B, C and D of red propolis were obtained by the technique of spray-dryer using colloidal silicon dioxide, pregelatinized starch and gelatin. Adjuvants, sodium starch glycolate and sodium carboxymethyl cellulose, were also used in the formulations. The dry extracts were evaluated for nebulized content of major labels (flavonoids) from própolis using HPLC-UV. The Liquid Chromatographic (LC) method consisted of a mobile phase of methanol:formic acid in a gradient system, RP-column (C18), temperature of oven (40°C) and UV detection at 281nm. Unifomity of content and dissolution method were used to assurance the quality of spray-dryer extract of red propolis. The HPLC method presented precision, accuracy and linearity for 4 majors from red propolis studied. Only the dried extracts A and D had flavonoid content close to 100%. The dried extracts B and C had flavonoid content of 68% and 131%. The nebulized dried extracts A and D were subjected to dissolution testing to assess the degree of solubilization of the markers of propolis in different pH range (1.0, 6.8 and 7.4) simulating the Gastrointestinal tract. The markers present in the dried extracts of propolis showed better solubility at pH around neutrality, with values of greater than 75% of dissolution after 120 minutes. There were influences of adjuvants in the dissolution velocity of the markers of propolis. The red propolis showed excellent activity against Pseudomonas aeruginosa Metalobetalactamase production. The compounds associated with this activity remained preserved even after the drying process. This technic can produce nutraceuticals.