Avaliação da Atividade Antimicrobiana e de Citotoxicidade in vitro e in vivo do extrato etanólico bruto do caule da Zeyheria tuberculosa (Vell) Bur. (Bignoniaceae): Perspectiva de um suplemento para Cicatrização de Feridas

Abstract

Numerous studies have been directed to the discovery of new agents, with healing action and / or antimicrobial, from plant extracts to be used as herbal teas, supplements or other natural ingredient that will bring benefits to the individual, even if they benefits are indirect. The aim of this study was to evaluate the antimicrobial and cytotoxic activities of ethanolic extract of the stem of the plant species for use Zeyheria tuberculous topically and / or oral ingestion as a healing of skin wounds. The extract was subjected to in vitro evaluation of antimicrobial activity by the disk diffusion technique, with strains ATCC (American Type Culture Collection) and the determination of Minimum Inhibitory Concentration (MIC). For in vivo testing was used as an animal model of Wistar rats. The animals were divided into six groups (n = 4) identified from the treatment. Daily dressings were carried out and evaluation of lesions and the clinical condition of the animal. In the 3rd, 7th, 11th and 14th days of the experiment were performed culture and biopsy of a lesion to perform histopathology. At the end of the experiment was collected blood for biochemical analysis and spleen and liver removed for histological analysis. Antimicrobial assays by disk diffusion showed activity against the strains of Streptococcus pyogenes, Staphylococcus epidermidis and Staphylococcus aureus, the latter being the minimum inhibitory concentration was 62.5 mg / mL. The extract acted significantly topically, by decreasing the initial inflammatory response. Histological analysis of the liver showed no hepatotoxicity. The biochemical evaluation parameters showed similar cholesterol, triglyceride and glucose among all animals in the experiment. Therefore shows promise for the development of a product that can be used alternatively for the repair of infected wounds.