Caracterização dos contaminantes fúngicos em leite de tanques de expansão produzidos no estado de Alagoas

Abstract

The objective of this study hold the count of yeasts and molds and characterize fungal contaminants of milk produced from expansion tanks in the State of Alagoas, was performed synergistically evaluating the sanitary conditions of these tanks. For collection of milk glass containers were used previously sterilized. Immediately after collection, the containers were packed in insulated boxes containing ice packs for later analysis in the laboratory of Food Inspection, Federal University of Alagoas. Sampling was performed in 23% of the 100 municipalities in community tanks of a cooperative state of Alagoas. The collections were made in the months of January, February and May 2013 In every moment 45 samples totaling 135 milk samples were collected. To perform the counting and identification of yeasts and molds used the methodology proposed by the APHA (American Public Health Association). The samples were plated on DRBC medium (agar Dichloran Rose Bengal Chloramphenicol). Was used in dilution 10-3. The plates were incubated at room temperature (25 ° C) for seven days. Of the 135 samples analyzed only 06 samples (4.44%) were not contaminated by yeasts and molds, 26 samples (19.26%) showed high contamination. More than a third of the samples 49/135 (36.3%) had an average of 3,58x105 CFU / ml of milk. Among the fungi isolated, stand out Penicillium spp. 18/135 (13.33%), Aspergillus spp. 85/145 (62.96%), Absidia sp 32/135 (23.70%), Fonsecaea sp 45/135 (33.33%) and Candida spp 19/135 (14.07%), being significantly greater than compared with those of other fungal isolates. Due to the high count of yeasts and molds, and the variety of its fungal contaminants found in this study suggest the adoption of efficient to reduce the multiplication of microorganisms, in order to avoid the problems caused by its metabolites means.