Estudo de semiquímicos que mediam a oviposição dos culicídeos aedes aegypti, aedes albopictus e culex quinquefasciatus

Abstract

Vectors-borne diseases are actually a great problem to the global public health, with approximately 500 million people being annually infected. An aggravating factor is the fact that many vectors showed increasing resistance to insecticides used in control programs. We present in this study results that showed a higher preference for oviposition by mosquito species Aedes aegypti, Aedes albopictus and Culex quinquefasciatus to organic infusions obtained from its headquarters plant and ether extracts. The development of this new control strategy aims at the rational use of insecticides, resulting in less damage to the environment; the extracts obtained by liquid-liquid extraction and stir bar sorption extraction were active in oviposition bioassays with mosquitoes A. aegypti and A. albopictus, enabling future identification of substances and determining their physiological activity by electroantennograph. It was first performed an acid-base extraction for the isolation and identification of substances in Aloe vera. The fractions that contain the neutral and phenolic compounds were the only ones which presented to be active for A. aegypti oviposition. We suggest, by GC-MS technique five compounds: Bicyclo [5.3.0] decapentaene (Azulene), (E)-2-Decenal, Heptadecane, 2,6-dibutyl-2,5-ciclohexadiene-1,4-dione e 4-methyl-2,6-ditert-butylphenol, but to confirm it’s necessary the comparison with their synthetic standards, and retention times and mass fragmentations besides electrophysiological studies of their relation to behavioral activities in insects. We also carried out preliminary studies of oviposition behavior of the mosquito C. quinquefasciatus by infusion aerobic chives, with promising results.