Investigação de atividade biológica em plantas medicinais usando speckle dinâmico

Abstract

We investigate in this work the bioactivity of leaves medicinal plants by means of the acquisition interferometric granulated images called Speckle pattern. When the coherent light (laser) focuses on a surface of a biologic environment, happen a optics phenomenon of interference called of biospeckle or dinamic speckle. For a environment biologic that is in activity, the incidents on the scattering centers undergo many interferences and become possible obtain informations about this system, as spatial as temporally, by biospeckle. We use methods of images analysis of the type Pearson's Correlation, in the which is observed the degree of similarity between the collected images, the matrices THSP (Temporal History of Speckle Pattern) and the MCO (Matrice of Co-ocorrence) to determine the probability of occurrence of intensities. For quantify the measure of dispersion of the poists around the main diagonal of the matrice MCO, the moment of inertia (MI) was used. Experimental arrangements to get the Speckle pattern by reflection objectively and subjectively were used in the monitoring of the thick leaf mint (Plectranthus amboinicus), balm (Melissa officinalis) and black mulberry leaf (Morus nigra). We investigate the bioactivity of these means like time function of collection (from freshly harvested leaf until its complete dehydration naturally). Based on the Akira Ishimaru theory for the scattering of a light beam that focuses on a rough surface, we used computational tools to simulate the behavior of correlation coefficient and adjust the simulations to the experimental datas for the measures of Pearson Correlation obtained in the three botanical specimens objects of study of this dissertation. A good agreement between simulation and experiment was observed with apparent evidence of bioactivity.