Microparticulas de própolis-polimero para aplicação como cosmético multifuncional

Abstract

The red propolis of state of Alagoas was highlighted by having a different chemical constitution and to outperform biological activities compared to other types of propolis, which was given geographical indication seal. Lately has been growing the search for products that promote skin protection, associating beyond the effect sunscreen other biological benefits, called multifunction products and among the many biological functions of propolis, we highlight those that provide skin protection. The microparticles facilitate manipulation of certain formulations where spray drying is one of methods to obtaining it. The objective was to develop microparticles propolis-polymer for evaluation of photoprotective activities, antioxidant, antimicrobial and healing in topical preparations. Was obtained hydroalcoholic extract and propolis fractions were the highlight compounds where identifying using HPLC. Was evaluated the Sun Protection Factor-SPF in vitro, antioxidant activity, healing activity in vivo, antimicrobial activity in vitro and toxicity in vivo. Were obtained microparticles by spray drying using biocompatible polymers. This microparticles were analyzed for markers, and characterization analyzes evaluating moisture content, particle size and Scanning Electron Microscope-SEM. The biological activities were also analyzed in microparticulate. The hydroalcoholic extract and propolis fractions were presented as a complex mixture of substances, which have been identified: gallic acid, feluric acid, daidizein, quercetin and formononetin. The Medium-High Polarity Fraction (MHFP) showed the best SPF 2,32 in 1% of concentration and it was better than controls in the healing tests. Topical treatment did not alter glycemic, lipid, renal profile, ion concentration, total proteins and fractions, alkaline phosphatase or lactate dehydrogenase. There was also no change of thyroid or pituitary hormones. The samples showed bacteriostatic/bactericidal and antioxidant activity. It was possible to obtain microparticles of MHFP with chitosan with smooth and uniform suface that was 1 e 3 μm with low moisture content. There was no loss in formononetin content for microparticulate and the biological activities have been preserved. Thus, it was possible to obtain microparticles of MHFP using chitosan polymer with photoprotective action, antioxidant, antimicrobial and wound healing which can be used for cosmetic purposes to prevent skin damage as a multifunctional product.