Primeiro relato do begomovírus Blainvillea yellow spot virus em quebra pedra (Phyllantus niruri)

Abstract

ABSTRACT

The Begomovirus genus is an important production limiting factor for several agricultural crops, due to its high degree of severity, geographic area and the number of species, which remains increasing. Its transmission is carried out by a complex of cryptic species of Bemisia tabaci, mainly the species MEAM1 (Middle East – Asia Minor 1) and, more recently, by seeds. In addition to affecting cultivated species, it has increasingly been reported to be present in uncultivated species, serving as a natural reservoir and the possible emergence of new species. The identification of species has contributed to decision making in the field, reducing the damage caused by these different pathogens. Thus, the objective of this study was to identify the species of Begomovirus infecting Phyllanthus niruri. Initially, identification at the genus level was performed using the universal primers PAL1v1978 (GCATCTGCAGGCCCACTYGTCTTYCCNGT) and PAR1c496 (AATACTGCAGGGCTTYCTRTACATRGG). The sequences obtained from the two clones BR_QPA_20A, BR_QPH_20A belong to isolates of Blainvillea yellow spot virus, having about 97% identity, according to BLASTn analyzes and paired comparisons using SDT (Sequence Demarcation Tool). In the phylogenetic relationship, it was observed that the internal components formed a clade with the isolates BR_QPA_20A, BR_QPH_20A, JX871392_BYSV, X871390_BYSV, corroborating and complementing that these isolates really belong to BYSV. Phyllanthus niruri is considered a host of Blainvillea yellow spot virus, being the first report in the world. New observations and field collections must be carried out to monitor the population growth of the species in the new host and in cultivated plants present in the area.

Keywords: Geminiviridae, uncultivated plants, species demarcation.