Avaliação da eficácia da operculina hamiltonii (operculina alata) na fase de proliferação da cicatrização de feridas cutâneas em camundongos

Abstract

Popularly the tincture of Jalapa Operculina hamiltonii (Operculina alata) can be used in skin diseases, however there is no scientific evidence about its effect on healing. Thus, this study investigated the effects of topical use of O. hamiltonii (tincture of Jalapa) in the proliferation phase of skin wounds. Adult male Swiss mice (9 weeks, n=5 per group, CEUA-UFAL: 047/2017) were anesthetized and an excisional skin lesion (Ø=1cm) was performed. They were photographed and received daily topical treatment with 50µL of saline or 5% hydroalcoholic solution (Controls) or in experimental groups with tincture of Jalapa (O. hamiltonii) commercial extract diluted to 1% (0.17 mg/mL) or pure (17 mg/mL). At the end of the experimental period, skin fragments containing the lesions were fixed, processed for inclusion in paraffin and stained in HE for histological analysis (qualitative). Animals remained healthy with no evidence of infection. Macroscopic examination of the wounds revealed edema, hyperemia, crusting in all groups and a significant reduction in the wound area at 6 days post-injury (proliferative phase) in relation to initial injury. In qualitative histological analysis, the experimental group treated with diluted O. hamiltonii extract (0.17 mg/mL) showed greater re-epithelialization of borders and an increase in small blood vessels compared to control groups. The results obtained suggest that topical application of the commercial extract tincture of Jalapa [O. hamiltonii] diluted (0.17 mg/mL) accelerates the proliferative phase with better re-epithelialization and formation of granulation tissue in wound healing in mice.