Análise metabolômica de águas fecais para caracterização de perfis bioquímicos de pacientes diagnósticados com doença inflamatória intestinal

Abstract

To understand the functioning of the metabolism of the intestinal microflora is extremely important for the planning of a potential biochemical profile, because the disturbances of metabolic faecal held by pathogenic microorganisms cause imbalance in the intestinal microflora generating the disbiose intestinal, benefitting from a frame of an inflammation of the Gastrointestinal tract (GIT). Currently, the diagnosis of Inflammatory Bowel Disease (IBD) is based on clinical features, endoscopic, histological and x-ray techniques, where the same, within such tools, which are invasive methods that generate discomfort to the patient. Due to the low sensitivity of the traditional examination, it becomes difficult in some situations, the precise diagnosis within two subtypes of the disease; Crohn's disease (CD) and Ulcerative Colitis (UC). Through the technique of Nuclear Magnetic Resonance of Hydrogen (NMR 1H) it is possible to analyse all the metabolites present in the fecal samples, in which combined with chemo metrics establishes the essential conditions for the extraction of the maximum of true information and accurately interpret the data generated by PCA (Principal component analysis), O-PLS-DA (Discriminant analysis of orthogonal projections to latente structures) and ANOVA (Analysis of variance) and in this way create the control Group (CG) to obtain a biochemical profile pattern and to compare with the profiles of the patients of the Hospital Universitário Prof. Alberto Antunes – HUPAA diagnosed with CD and UC. The analysis of the metabolômica of biomarkers related to disease, in addition to being a practical non-invasive, provides us with the creation of a powerful and effective method with a high sensitivity for the diagnosis of patients with CD and UC.