Nitrato de prata e tiossulfato de prata como inibidores da ação do etileno em cultivo in vitro de Annona glabra L.

Abstract

Annona glabra L., a tropical fruit species belonging to the Annonaceae family, has attracted interest from researchers because of its adaptive capacity to extreme environments, having great potential as a rootstock for species of the same genus. One of the difficulties reported in the literature regarding the sexual propagation of this species is the slow germination process, with marked unevenness. Asexual propagation methods, such as in vitro cultivation, have some limitations, among them, the accumulation of ethylene in the microenvironment formed inside the flask, causing early leaf abscission in the explants. This work aimed to study two inhibitors of the action of ethylene, silver nitrate and silver thiosulfate on in vitro cultivation of Annona glabra L. The explants were obtained from young vegetative branches of 3-year-old mother plants grown in the nursery of Plant Biotechnology, Center for Agricultural Sciences, Federal University of Alagoas. The experiments were performed in a completely randomized design, consisting of 4 treatments and 20 replications. The culture medium used in both experiments was MS supplemented with 30g L-1

sucrose and 7g L-1

agar. The concentrations tested were: 0;

0.2; 2.0 and 20.0 μmol L -1

of silver nitrate and silver thiosulfate respectively, added to MS medium. The ethylene action inhibitor, silver thiosulfate, was more efficient to reduce early leaf abscission in the in vitro establishment of the species under study.