Estudo do potencial cicatrizante, antimicrobiano e antiedematogênico da Musa paradisíaca L.

Abstract

This study is a preclinical research on the use of banana (Musa paradisiacal L.), whose main objective was to evaluate the antimicrobial potential, antiedematogenic, healing and cell viability of the extracts of the leaves and pseudostem of Musa paradisiacal L. Extracts were listed according to the plant part used, Musa 1 (ethanol extract of the leaf), Musa 2 (aqueous extract of the leaf), Musa 3 (ethanol extract pseudostem) and Musa 4 (aqueous extract pseudostem). In vitro tests were conducted cell viability by MTT method - [bromide 3 - (4,5 - dimethylthiazol - 2 - yl) tetrazolium] and for antimicrobial disk diffusion and agar drilling. For in vivo tests were used 36 Wistar rats in the experiment healing and 36 mice in Swis ear edema induced by capsaicin, provided by the Central Animal Laboratory Creation of Federal University of Alagoas, and handled in accordance with standards established by the Commission on Ethics Use of Laboratory Animals. It was found in testing antibiotics on the bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and the yeast Candida albicans, the ethanol and aqueous extracts of Musa paradisiacal L. do not inhibit microbial growth. The test was conducted cell viability at concentrations of 100, 200, 1000 and 2000 μg/mL, and showed that extracts at a concentration of 200 μg/mL did not induce cell toxicity in J774 macrophage lineage and in other concentrations there is a variation of cytotoxicity in extracts. The extracts were also tested in vivo experiments to evaluate the potential healing and antiedematogenic leaves and pseudostem of banana. It was found that no significant inhibition of ear edema formation induced by capsaicin, indicating that the extract has anti-inflammatory site. As for the healing of wounds clean, it was found that the extracts have healing action inducing epithelialization of the wound better than the positive control.