Avaliação da atividade e purificação parcial de um inibidor de tripsina presente na larva do inseto Tribolium castaneum

Abstract

Enzyme inhibitors are molecules that have the ability to decrease or interrupt the catalytic activity of enzymes, many of them are produced endogenously for the purpose to maintain the organism's homeostasis. Trypsin inhibitors have been studied due to their importance in prague control, to understand the mechanisms of inhibition and even as a possibility for new drugs. The objective of this work was to evaluate the activity of an endogenous trypsin inhibitor of the Tribolium castaneum larva by means of a partial purification. The first stage consisted of a salting out with ammonium sulfate, the second stage used an ultrafiltration membrane. After saline precipitation and ultrafiltration, the larvae extract had a decrease in contaminants confirmed by a SDS-PAGE polyacrylamide gel, showing a partial purification and managed to inhibit about 58% of bovine trypsin activity, when the sample was subjected to high temperatures, its activity remained conserved. With these results we were able to prove the presence of an endogenous trypsin inhibitor in the T. castaneum larva, which can be used for future biotechnological applications such as disease treatment.