Potêncial biotenológico dos derivados de aminoguanidina na inibição dos estresses oxidativo e carbonílico

Abstract

The consumption of sugars has been progressively increasing over the last few decades, due to the intake of processed foods from fast food chains, soft drinks, among others. Based on this, the accumulation of reactive oxygen species and reactive carbonyl species has grown proportionally to the consumption. These species have action mechanisms capable of causing irreversible changes in protein structures and various biomolecules, causing oxidative and carbonyl stress. The purpose of this study is to investigate the inhibitory potential of glycation and the antioxidant potential of aminoguanidine derivatives. The antioxidant capacity of the aminoguanidine derivatives was measured by capturing hypochlorous acid (HOCl) and superoxide radical anion (O2 •−). The antiglycation evaluation was measured both in the initial stages by glycation of bovine serum albumin (BSA) in the presence of glucose/fructose and in the intermediate stage by capturing methylglyoxal. All derivatives showed antioxidant capacity; however, they are more active against HOCl, with emphasis on LQMs 55, 103, 130, and 137, which showed the best results in the two antioxidant assays applied. Of these, the two most promising are LQM 55 and LQM 137, as they also showed the best results for the antiglycation assays, with IC50 values of 60.5 and 33.6 µM for the initial stage and 70.5 and 65.3 for the intermediate stage of glycation, respectively. Thus, samples LQM 55 and LQM 137 have greater potential for acting against carbonyl and oxidative stress, however, further studies should be carried out, including those related to sample toxicity