Perfil de proteínas expressas em amostras de saliva de indivíduos com câncer de boca através da técnica de SDS-PAGE eletrofores

Abstract

It is estimated that by 2030, there will be 20.3 million new cases of cancer and 13.2 million deaths annually (PATIL. Et. Al. 2015). Mouth cancer (CB) next to pharyngeal cancer occupies the 6th position in incidence in the world. The estimated annual rate for CB is around 275 million (WARNAKULASURIYA. 2009). The purpose of the study is to analyze the protein expressions in CB saliva and control using the SDS-PAGE within the range determined by the marker used in the study in the range: 300 to 40 kDA, and to compare the patterns of the bands expressed in the gel with the bioinformatics data performed on the UNIPROT platform. The results obtained were promising in a way that it was possible to visualize the protein profiles among study participants. It was observed that in CB there is an exacerbated protein expression (n = 50) in relation to the control (n = 44), and bioinformatics still points out that there are no proteins in common between CB and healthy individuals, in addition, only in patients it is It is possible to visualize proteins in the range between 70 and 50 kDa. All of these factors indicate that there is a loss in the regulatory mechanisms that control homeostasis. With the advancement of technology applied to health, it is possible to think that in the future, molecular analyzes will become more accessible and common, helping to discover some pathologies still in their initial stages, such as the cancers addressed in this study, and assisting in the clinical prognosis of patients already diagnosed.